Identification of major factors influencing ELISpot-based monitoring of cellular responses to antigens from Mycobacterium tuberculosis.

Journal article

A number of different interferon-gamma ELISpot protocols are in use in laboratories studying antigen-specific immune responses. It is therefore unclear how results from different assays compare, and what factors most significantly influence assay outcome. One such difference is that some laboratories use a short in vitro stimulation period of cells before they are transferred to the ELISpot plate; this is commonly done in the case of frozen cells, in order to enhance assay sensitivity. Other ...

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Authors: Smith, S; Joosten, SA; Verscheure, V; Pathan, A; Mcshane, H

• Publication status: Published
• Journal: PloS one
• Volume: 4
• Issue: 11
• Pages: e7972
• Publication date: 2009-01-01
• DOI: 10.1371/journal.pone.0007972
• EISSN: 1932-6203
• ISSN: 1932-6203
• Language: English
• Keywords: Tuberculosis Vaccines; Chemistry, Clinical; Reproducibility of Results; Leukocytes, Mononuclear; Automation; Humans; Sensitivity and Specificity; Enzyme-Linked Immunosorbent Assay; Antigens, Bacterial; Software; Time Factors; Clinical Laboratory Techniques; Centrifugation, Density Gradient; Mycobacterium tuberculosis; Cryopreservation