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Journal article

Identification of major factors influencing ELISpot-based monitoring of cellular responses to antigens from Mycobacterium tuberculosis.

Abstract:

A number of different interferon-gamma ELISpot protocols are in use in laboratories studying antigen-specific immune responses. It is therefore unclear how results from different assays compare, and what factors most significantly influence assay outcome. One such difference is that some laboratories use a short in vitro stimulation period of cells before they are transferred to the ELISpot plate; this is commonly done in the case of frozen cells, in order to enhance assay sensitivity. Other ...

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Publication status:
Published

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Publisher copy:
10.1371/journal.pone.0007972

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Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Jenner Institute
Role:
Author
Journal:
PloS one More from this journal
Volume:
4
Issue:
11
Pages:
e7972
Publication date:
2009-01-01
DOI:
EISSN:
1932-6203
ISSN:
1932-6203

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