Voltage-activated Na+ currents and their suppression by phorbol ester in clonal insulin-producing RINm5F cells.

Journal article

The whole-cell configuration of the patch-clamp technique was applied on the clonal insulin-producing cell line RINm5). Thus attempts were made to characterize voltage-activated inward and outward membrane currents and to examine to what extent these were affected by both long-term and acute exposure to the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA). Current responses to voltage-clamp steps up to -40 mV were small. A pulse to -28 mV evoked an inward current, and slowly activatin...

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Authors: Rorsman, P; Arkhammar, P; Berggren, P

• Publication status: Published
• Journal: American journal of physiology
• Volume: 251
• Issue: 6 Pt 1
• Pages: C912-C919
• Publication date: 1986-12-01
• ISSN: 0002-9513
• Language: English
• Keywords: Animals; Insulinoma; Adenoma, Islet Cell; Sodium; Cell Line; Membrane Potentials; Pancreatic Neoplasms; Action Potentials; Calcium; Clone Cells; Insulin; Ion Channels; Tetradecanoylphorbol Acetate; Rats