Journal article icon

Journal article

Comparison of the N-linked glycans from soluble and GPI-anchored CD59 expressed in CHO cells.

Abstract:

The N-linked glycosylation of recombinant human CD59, expressed in Chinese hamster ovary (CHO) cells with and without a membrane anchor, was compared to examine the effect of the anchor on glycan processing. N-Linked glycans were released with peptide-N-glycosidase F (PNGase F) within gel from SDS-PAGE-isolated soluble and glycosylphosphatidylinositol (GPI)-anchored human CD59 expressed in CHO cells. The anchored form contained core-fucosylated neutral and sialylated bi-, tri-, and tetraanten...

Expand abstract
Publication status:
Published

Actions


Access Document


Publisher copy:
10.1093/glycob/12.4.261

Authors


Wheeler, SF More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, RDM, Molecular Medicine
Harvey, DJ More by this author
Journal:
Glycobiology
Volume:
12
Issue:
4
Pages:
261-271
Publication date:
2002-04-05
DOI:
EISSN:
1460-2423
ISSN:
0959-6658
URN:
uuid:60c744a4-3950-46be-93a9-558f6e39eb85
Source identifiers:
20519
Local pid:
pubs:20519

Terms of use


Metrics



If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP