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Next Generation Sequencing (NGS) reveals casing microbiome dynamics during button mushroom cultivation: implications for dry and wet bubble diseases

Subtitle:
Next Generation Sequencing (NGS) reveals casing microbiome dynamics during button mushroom cultivation

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Department:
Department of Plant Sciences
Role:
Creator
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Grant:
2016-IDD-00013
Funding agency for:
Project
Publisher:
University of Oxford
Publication date:
2018
Spatial coverage:
La Rioja (Spain)
Language:
English
Subjects:
Format:
Digital
Documentation:
Compost, casing and basidiome samples analyzed in this study were obtained in triplicate from an Agaricus bisporus crop trial by taking samples along the crop cycle. DNA was extracted, purified and metagenomic analysis was performed by sequencing bacterial and fungal libraries through Illumina MiSeq. Bioinformatics: The quality of the raw reads was assessed using FastQC. The raw reads were trimmed, filtered with a Phred quality score of at least 30 and all adapters removed with Trim Galore. ... Expand documentation

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