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Validating a fully automated real-time PCR-based system for use in the molecular diagnostic analysis of colorectal carcinoma: a comparison with NGS and IHC

Abstract:

Molecular testing is increasingly needed in colorectal carcinoma (CRC) and the current clinically relevant mutations are in BRAF, KRAS and NRAS. This study aimed to further validate a new alternative polymerase chain reaction (PCR) platform (Idylla, Biocartis) against existing next-generation sequencing (NGS) and immunohistochemistry (IHC) assays.56 Idylla tests were performed on 43 CRC cases, in a total of 74 comparisons against an NGS panel (Ion Torrent) and the VE1 (anti-BRAF) antibody IHC...

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Publication status:
Published
Peer review status:
Peer reviewed

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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Surgical Sciences
ORCID:
0000-0001-6344-9081
More by this author
Institution:
University of Oxford
Division:
NDORMS
Department:
KIR
More by this author
Institution:
University of Oxford
Division:
RDM
Department:
RDM Clinical Laboratory Sciences
Publisher:
BMJ Publishing Group Publisher's website
Journal:
Journal of Clinical Pathology Journal website
Volume:
70
Issue:
7
Publication date:
2017-03-14
Acceptance date:
2017-02-13
DOI:
EISSN:
1472-4146
ISSN:
0021-9746
Pubs id:
pubs:686316
URN:
uri:6037ef38-c86f-4c97-b3c5-da3ffecffd5c
UUID:
uuid:6037ef38-c86f-4c97-b3c5-da3ffecffd5c
Local pid:
pubs:686316
Language:
English

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