Human CD1d-glycolipid tetramers generated by in vitro oxidative refolding chromatography.

Journal article

CD1 molecules are specialized in presenting lipids to T lymphocytes, but identification and isolation of CD1-restricted lipid specific T cells has been hampered by the lack of reliable and sensitive techniques. We here report the construction of CD1d-glycolipid tetramers from fully denatured human CD1d molecules by using the technique of oxidative refolding chromatography. We demonstrate that chaperone- and foldase-assisted refolding of denatured CD1d molecules and beta(2)-microglobulin in th...

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Authors: Karadimitris, A; Gadola, S; Altamirano, M; Brown, D; Woolfson, A

• Publication status: Published
• Journal: Proceedings of the National Academy of Sciences of the United States of America
• Volume: 98
• Issue: 6
• Pages: 3294-3298
• Publication date: 2001-03-01
• DOI: 10.1073/pnas.051604498
• EISSN: 1091-6490
• ISSN: 0027-8424
• Language: English
• Keywords: Antigens, CD1d; Liver Cirrhosis; Protein Folding; Ceramides; Hepatitis C; Antigens, CD1; Mice, Knockout; Staining and Labeling; Cells, Cultured; Oxidation-Reduction; Mice; Glycolipids; Animals; Humans; Ligands