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Conformational transitions in DNA polymerase I revealed by single-molecule FRET.

Abstract:

The remarkable fidelity of most DNA polymerases depends on a series of early steps in the reaction pathway which allow the selection of the correct nucleotide substrate, while excluding all incorrect ones, before the enzyme is committed to the chemical step of nucleotide incorporation. The conformational transitions that are involved in these early steps are detectable with a variety of fluorescence assays and include the fingers-closing transition that has been characterized in structural st...

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Publication status:
Published

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Publisher copy:
10.1073/pnas.0910909107

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Institution:
University of Oxford
Department:
Oxford, MPLS, Physics
Role:
Author
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Journal:
Proceedings of the National Academy of Sciences of the United States of America
Volume:
107
Issue:
2
Pages:
715-720
Publication date:
2010-01-05
DOI:
EISSN:
1091-6490
ISSN:
0027-8424
URN:
uuid:60029ada-9363-458d-bea6-941a302273f5
Source identifiers:
46152
Local pid:
pubs:46152

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