The application of aqueous two-phase systems to oligosaccharide synthesis by alpha-mannosidase catalysed glycosyl transfer reactions.

Journal article

Aqueous two-phase systems formed from PEG and dextran have been applied to the synthesis of oligosaccharide by Jack bean alpha-mannosidase in reverse. Whilst rates of synthesis and percentage yields were similar in two-phase systems and one-phase aqueous buffer systems, a ten-fold increase in yield of product per unit of enzyme was seen. In addition, the use of aqueous two-phase systems offers potential process advantages over one-phase systems for the synthesis of oligosaccharides.

Authors: Bartlett, T; Rastall, R; Rees, N; Adlard, M; Bucke, C

• Publication status: Published
• Journal: Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986)
• Volume: 55
• Issue: 1
• Pages: 73-78
• Publication date: 1992-01-01
• DOI: 10.1002/jctb.280550112
• EISSN: 1097-4660
• ISSN: 0268-2575
• Language: English
• Keywords: Solubility; Oligosaccharides; Kinetics; Evaluation Studies as Topic; Mannosidases; Polyethylene Glycols; Molecular Sequence Data; alpha-Mannosidase; Glycosides; Carbohydrate Sequence; Dextrans; Water