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The application of aqueous two-phase systems to oligosaccharide synthesis by alpha-mannosidase catalysed glycosyl transfer reactions.

Abstract:
Aqueous two-phase systems formed from PEG and dextran have been applied to the synthesis of oligosaccharide by Jack bean alpha-mannosidase in reverse. Whilst rates of synthesis and percentage yields were similar in two-phase systems and one-phase aqueous buffer systems, a ten-fold increase in yield of product per unit of enzyme was seen. In addition, the use of aqueous two-phase systems offers potential process advantages over one-phase systems for the synthesis of oligosaccharides.
Publication status:
Published

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Publisher copy:
10.1002/jctb.280550112

Authors


Bartlett, TJ More by this author
Rastall, RA More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Inorganic Chemistry
Adlard, MW More by this author
Journal:
Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986)
Volume:
55
Issue:
1
Pages:
73-78
Publication date:
1992
DOI:
EISSN:
1097-4660
ISSN:
0268-2575
URN:
uuid:5fe80f04-3ef9-469d-bf27-ebc838b635f5
Source identifiers:
115957
Local pid:
pubs:115957

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