Structural basis for phosphatidylinositol phosphate kinase type Igamma binding to talin at focal adhesions.

Journal article

The cytoskeletal protein talin binds to a short C-terminal sequence in phosphatidylinositol phosphate kinase type Igamma (PIPKIgamma), activating the enzyme and promoting the local production of phosphatidylinositol 4,5 bisphosphate, which regulates focal adhesion dynamics as well as clathrin-mediated endocytosis in neuronal cells. Here we show by crystallographic, NMR, and calorimetric analysis that the phosphotyrosine binding (PTB)-like domain of talin engages the PIPKIgamma C terminus in a...

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Authors: de Pereda, J; Wegener, K; Santelli, E; Bate, N; Ginsberg, M

• Journal: Journal of biological chemistry
• Volume: 280
• Issue: 9
• Pages: 8381-8386
• Publication date: 2005-03-01
• DOI: 10.1074/jbc.m413180200
• EISSN: 1083-351X
• ISSN: 0021-9258
• Language: English
• Keywords: Integrin beta Chains; Crystallography, X-Ray; Magnetic Resonance Spectroscopy; Calorimetry; Mice; Phosphorylation; Binding Sites; Animals; Tyrosine; Protein Conformation; Focal Adhesions; Talin; Protein Binding; Salts; Phosphotransferases (Alcohol Group Acceptor); Protein Structure, Tertiary; Peptides; Endocytosis; Models, Molecular; Amino Acid Motifs; Gene Deletion; DNA, Complementary