Proteome analysis of rhoptry-enriched fractions isolated from Plasmodium merozoites.

Journal article

The rhoptries of Plasmodium species participate in merozoite invasion and modification of the host erythrocyte. However, only a few rhoptry proteins have been identified using conventional gene identification protocols. To investigate the protein organization of this organelle and to identify new rhoptry proteins, merozoite rhoptries from three different Plasmodium rodent species were enriched by sucrose density gradient fractionation, and subjected to proteome analysis using multidimensional...

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Authors: Sam-Yellowe, T; Florens, L; Wang, T; Raine, J; Carucci, D

• Journal: Journal of proteome research
• Volume: 3
• Issue: 5
• Pages: 995-1001
• Publication date: 2004-01-01
• DOI: 10.1021/pr049926m
• EISSN: 1535-3907
• ISSN: 1535-3893
• Language: English
• Keywords: Peptide Hydrolases; Membrane Proteins; Protozoan Proteins; Repetitive Sequences, Nucleic Acid; Plasmodium; Plasmodium chabaudi; Cell Fractionation; Animals; Plasmodium berghei; Plasmodium yoelii; Mass Spectrometry; Endopeptidases; Databases, Protein; Protein Sorting Signals; Proteome