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In vitro degradation of articular cartilage: does trypsin treatment produce consistent results?

Abstract:

It is common practice in laboratories to create models of degraded articular cartilage in vitro and use these to study the effects of degeneration on cartilage responses to external stimuli such as mechanical loading. However, there are inconsistencies in the reported action of trypsin, and there is no guide on the concentration of trypsin or the time to which a given sample can be treated so that a specific level of proteoglycan depletion is achieved. This paper argues that before any level ...

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Journal:
Journal of anatomy
Volume:
209
Issue:
2
Pages:
259-267
Publication date:
2006-08-05
DOI:
EISSN:
1469-7580
ISSN:
0021-8782
URN:
uuid:5f57568b-4be9-4c2f-b5a1-e37cb579204d
Source identifiers:
371138
Local pid:
pubs:371138

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