The A167Y mutation converts the herpes simplex virus type 1 thymidine kinase into a guanosine analogue kinase.

Journal article

The thymidine (dThd) kinase (TK) encoded by herpes simplex virus type 1 (HSV-1) is not only endowed with dThd kinase, but also with thymidylate (dTMP) kinase and 2'-deoxycytidine (dCyd) kinase (dCK) activity. HSV-1 TK also recognizes a variety of antiherpetic guanine nucleoside analogues such as acyclovir (ACV), ganciclovir (GCV), lobucavir (LBV), penciclovir (PCV), and others (i.e., A5021). Site-directed mutagenesis of the highly conserved Ala-167 to Tyr in HSV-1 TK completely abolished TK, ...

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Authors: Balzarini, J; Liekens, S; Esnouf, R; De Clercq, E

• Publication status: Published
• Journal: Biochemistry
• Volume: 41
• Issue: 20
• Pages: 6517-6524
• Publication date: 2002-05-01
• DOI: 10.1021/bi0255930
• EISSN: 1520-4995
• ISSN: 0006-2960
• Language: English
• Keywords: Kinetics; Pyrimidine Nucleosides; Phosphorylation; Substrate Specificity; Alanine; Purine Nucleosides; Enzyme Inhibitors; Thymidine Kinase; Purine Nucleotides; Phosphotransferases (Alcohol Group Acceptor); Amino Acid Substitution; Herpesvirus 1, Human; Mutagenesis, Site-Directed; Tyrosine; Antiviral Agents; Antineoplastic Agents