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Angiotensin-converting enzyme inhibition studies by natural leech inhibitors by capillary electrophoresis and competition assay.

Abstract:

A protocol to follow the processing of angiotensin I into angiotensin II by rabbit angiotensin-converting enzyme (ACE) and its inhibition by a novel natural antagonist, the leech osmoregulator factor (LORF) using capillary zonal electrophoresis is described. The experiment was carried out using the Beckman PACE system and steps were taken to determine (a) the migration profiles of angiotensin and its yielded peptides, (b) the minimal amount of angiotensin II detected, (c) the use of different...

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Authors


Deloffre, L More by this author
Sautiere, PE More by this author
Huybrechts, R More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Physiology Anatomy and Genetics
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Journal:
European journal of biochemistry / FEBS
Volume:
271
Issue:
11
Pages:
2101-2106
Publication date:
2004-06-05
DOI:
EISSN:
1432-1033
ISSN:
0014-2956
URN:
uuid:5eaf1ef4-b8ac-4de9-b022-48e7bb2d2e25
Source identifiers:
345832
Local pid:
pubs:345832

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