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Fast-atom-bombardment mass spectrometry. A new technique for the determination of molecular weights and amino acid sequences of peptides.

Abstract:

A detailed study of the mass spectra of peptides produced by the new technique of fast-atom bombardment is reported. Molecular weights of unmodified peptides containing up to 21 amino acids have been determined. In favourable cases, the molecular-weight determination may be made on as little as 0.1 nmol of sample. Positive-ion and negative-ion spectra are obtained with equal facility. With sample sizes in the range 2-50nmol, sequence information can be obtained in many cases. The technique re...

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Publication status:
Published

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Journal:
The Biochemical journal
Volume:
201
Issue:
1
Pages:
105-117
Publication date:
1982-01-05
EISSN:
1470-8728
ISSN:
0264-6021
URN:
uuid:5e8be1bc-f575-4f5e-a565-64a9e6ebda6a
Source identifiers:
59469
Local pid:
pubs:59469

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