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The presence of outer arm fucose residues on the N-glycans of tissue inhibitor of metalloproteinases-1 reduces its activity.

Abstract:

Tissue inhibitor of metalloproteinases-1 (TIMP-1) inhibits matrix metalloproteinases (MMPs) by binding at a 1:1 stoichiometry. Here we have shown the involvement of N-glycosylation in the MMP inhibitory ability of TIMP-1. TIMP-1, purified from HEK 293 cells overexpressing TIMP-1 (293 TIMP-1), showed less binding and inhibitory abilities to MMPs than TIMP-1 purified from fibroblasts or SF9 insect cells infected with TIMP-1 baculovirus. Following deglycosylation of TIMP-1, all forms of TIMP-1 s...

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Publication status:
Published

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Publisher copy:
10.1021/pr400276r

Authors


Saldova, R More by this author
Harvey, DJ More by this author
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Journal:
Journal of proteome research
Volume:
12
Issue:
8
Pages:
3547-3560
Publication date:
2013-08-05
DOI:
EISSN:
1535-3907
ISSN:
1535-3893
URN:
uuid:5e6a327b-56a2-4db7-b5a6-82034e55a80b
Source identifiers:
411134
Local pid:
pubs:411134

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