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Carbene footprinting reveals binding interfaces of a multimeric membrane‐spanning protein

Abstract:

Mapping the interaction sites between membrane‐spanning proteins is a key challenge in structural biology. In this study a carbene‐footprinting approach was developed and applied to identify the interfacial sites of a trimeric, integral membrane protein, OmpF, solubilised in micelles. The diazirine‐based footprinting probe is effectively sequestered by, and incorporated into, the micelles, thus leading to efficient labelling of the membrane‐spanning regions of the protein upon irradiation at ...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1002/anie.201708254

Authors


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ORCID:
0000-0003-3661-5173
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ORCID:
0000-0002-1667-9690
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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Biochemistry
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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Biochemistry
ORCID:
0000-0002-3273-0302
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University of Nottingham More from this funder
Publisher:
Wiley‐VCH Verlag Publisher's website
Journal:
Angewandte Chemie (International ed. in English) Journal website
Volume:
56
Issue:
47
Pages:
14873-14877
Publication date:
2017-10-20
Acceptance date:
2017-09-27
DOI:
EISSN:
1521-3773
ISSN:
1433-7851
Pubs id:
pubs:732518
URN:
uri:5d945bf0-6e65-4c89-b0eb-bf351569c682
UUID:
uuid:5d945bf0-6e65-4c89-b0eb-bf351569c682
Local pid:
pubs:732518

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