Expression of voltage-dependent calcium channels in the embryonic rat midbrain.
The diversity of expression of high-voltage activated voltage-dependent calcium channels (VDCC) was investigated with whole-cell voltage-clamp recordings from dissociated embryonic rat ventral mesencephalic cells over a 7-day culture period. Cell phenotype was identified post-recording by fluorescent immunocytochemistry as tyrosine hydroxylase positive (TH+) or glutamic acid decarboxylase positive (GAD+). Both TH+ and GAD+ cells displayed high-threshold calcium (Ca(2+)) currents activated by ...Expand abstract
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