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Mass spectrometry defines the stoichiometry of ribosomal stalk complexes across the phylogenetic tree.

Abstract:

The ribosomal stalk complex plays a crucial role in delivering translation factors to the catalytic site of the ribosome. It has a very similar architecture in all cells, although the protein components in bacteria are unrelated to those in archaea and eukaryotes. Here we used mass spectrometry to investigate ribosomal stalk complexes from bacteria, eukaryotes, and archaea in situ on the ribosome. Specifically we targeted ribosomes with different optimal growth temperatures. Our results showe...

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Publication status:
Published

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Publisher copy:
10.1074/mcp.m000072-mcp201

Authors


Journal:
Molecular and cellular proteomics : MCP
Volume:
9
Issue:
8
Pages:
1774-1783
Publication date:
2010-08-01
DOI:
EISSN:
1535-9484
ISSN:
1535-9476
Language:
English
Keywords:
Pubs id:
pubs:59340
UUID:
uuid:5c8c349e-2cd5-4384-8a13-07ecaa568996
Local pid:
pubs:59340
Source identifiers:
59340
Deposit date:
2012-12-19

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