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Functional expression of the alpha-hemolysin of Staphylococcus aureus in intact Escherichia coli and in cell lysates. Deletion of five C-terminal amino acids selectively impairs hemolytic activity.

Abstract:

The alpha-hemolysin gene from Staphylococcus aureus, excluding the 5' region encoding the hydrophobic leader sequence, was amplified from genomic DNA. The identity of the disputed C terminus has been confirmed and revisions made to the internal sequence. The hemolysin is expressed at high levels in Escherichia coli and has been purified to homogeneity from this source. In addition, active [35S-Met]alpha-hemolysin of high specific radioactivity can be generated in an E. coli transcription-tran...

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Publication status:
Published

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Authors


Krishnasastry, M More by this author
Kasianowicz, J More by this author
Journal:
The Journal of biological chemistry
Volume:
267
Issue:
15
Pages:
10902-10909
Publication date:
1992-05-05
EISSN:
1083-351X
ISSN:
0021-9258
URN:
uuid:5c6b2478-3c56-4150-84a0-43524896c0ef
Source identifiers:
52227
Local pid:
pubs:52227

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