Journal article

Fluorescence-based active site probes for profiling deubiquitinating enzymes.

Abstract:: Novel ubiquitin-based active site probes including a fluorescent tag have been developed and evaluated. A new, functionalizable electrophilic trap is utilized allowing for late stage diversification of the probe. Attachment of fluorescent dyes allowed direct detection of endogenous deubiquitinating enzyme (DUB) activities in cell extracts by in-gel fluorescence imaging.

Publication status:: Published

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APA Style

McGouran, J., Kramer, H., Mackeen, M., di Gleria, K., Altun, M., & Kessler, B. (2012). Fluorescence-based active site probes for profiling deubiquitinating enzymes. Organic and Biomolecular Chemistry, 10(17), 3379–3383.

MLA Style

McGouran, J., et al. “Fluorescence-Based Active Site Probes for Profiling Deubiquitinating Enzymes.” Organic and Biomolecular Chemistry, vol. 10, no. 17, 2012, pp. 3379–83.

Chicago Style

McGouran, J, H Kramer, M Mackeen, K di Gleria, M Altun, and B Kessler. 2012. “Fluorescence-Based Active Site Probes for Profiling Deubiquitinating Enzymes.” Organic and Biomolecular Chemistry 10 (17): 3379–83.
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Publisher copy:: 10.1039/c2ob25258a

Authors

+ McGouran, J More by this author

Role:: Author

+ Kramer, H More by this author

Role:: Author

+ Mackeen, M More by this author

Role:: Author

+ di Gleria, K More by this author

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+ Altun, M More by this author

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Journal:: Organic and biomolecular chemistry More from this journal
Volume:: 10
Issue:: 17
Pages:: 3379-3383
Publication date:: 2012-05-01
DOI:: 10.1039/c2ob25258a
EISSN:: 1477-0539
ISSN:: 1477-0520

Language:: English
Keywords:: Fluorescent Dyes

Models, Molecular

Enzyme Assays

Click Chemistry

Catalytic Domain

HEK293 Cells

Endopeptidases

Humans

Ubiquitin
Pubs id:: pubs:320456
UUID:: uuid:5c653a22-c91e-4b24-9baf-a700e4729434
Local pid:: pubs:320456
Source identifiers:: 320456
Deposit date:: 2012-12-19

Terms of use

Copyright date:: 2012

Licence:: Terms and Conditions of Use for Oxford University Research Archive

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