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Structure of isopenicillin N synthase complexed with substrate and the mechanism of penicillin formation.

Abstract:

The biosynthesis of penicillin and cephalosporin antibiotics in microorganisms requires the formation of the bicyclic nucleus of penicillin. Isopenicillin N synthase (IPNS), a non-haem iron-dependent oxidase, catalyses the reaction of a tripeptide, delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-valine (ACV), and dioxygen to form isopenicillin N and two water molecules. Mechanistic studies suggest the reaction is initiated by ligation of the substrate thiolate to the iron centre, and proceeds thro...

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Publication status:
Published

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Publisher copy:
10.1038/42990

Authors


Clifton, IJ More by this author
Hensgens, CM More by this author
Shibata, N More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Organic Chemistry
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Journal:
Nature
Volume:
387
Issue:
6635
Pages:
827-830
Publication date:
1997-06-05
DOI:
EISSN:
1476-4687
ISSN:
0028-0836
URN:
uuid:5bb82b22-a043-4381-8476-98f3f54617d9
Source identifiers:
36059
Local pid:
pubs:36059

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