Paradoxical effects of trichostatin A: inhibition of NF-Y-associated histone acetyltransferase activity, phosphorylation of hGCN5 and downregulation of cyclin A and B1 mRNA.

Journal article

Trichostatin A (TSA), an inhibitor of histone deacetylase (HDAC), is widely used to study the role of histone acetylation in gene expression, since genes that use histone acetylation as a means of regulating expression may be up regulated when TSA is added. In this study, however, we show that TSA has an unexpected paradoxical effect leading to inhibition of NF-Y-associated histone acetyl transferase (HAT) activity and phosphorylation of the HAT, hGCN5. TSA treatment of cells resulted in dimi...

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Authors: Nair, A; Boersma, L; Schiltz, L; Chaudhry, M; Muschel, R

• Publication status: Published
• Journal: Cancer letters
• Volume: 166
• Issue: 1
• Pages: 55-64
• Publication date: 2001-05-01
• DOI: 10.1016/s0304-3835(01)00418-9
• EISSN: 1872-7980
• ISSN: 0304-3835
• Language: English
• Keywords: Cyclin A; Down-Regulation; Transcription Factors; CCAAT-Binding Factor; Hela Cells; Saccharomyces cerevisiae Proteins; Histone Acetyltransferases; Cell Cycle Proteins; Cyclin B; Phosphorylation; Enzyme Inhibitors; Transcription, Genetic; Hydroxamic Acids; Transfection; Humans; Trans-Activators; Acetyltransferases; RNA, Messenger; p300-CBP Transcription Factors