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An evaluation of Coomassie Brilliant Blue as a stain for quantitative microdensitometry of protein in section.

Abstract:

The specificity and stoichiometry of the binding of Coomassie Brilliant Blue (CBB) to protein in section has been examined using both frozen protein matrices and plant material. The maximum adsorbance of the stain, bound and in solution, was found to be 620 nm although variation in the results at this wavelength necessitated measurements to be made at 600 nm. After enzyme treatments of sectioned plant material embedded in resin, all CBB-binding biological material was shown to be sensitive to...

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Publication status:
Published

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Publisher copy:
10.1177/26.8.80420

Authors


Cawood, AH More by this author
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Institution:
University of Oxford
Department:
Oxford, MPLS, Plant Sciences
Journal:
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
Volume:
26
Issue:
8
Pages:
645-650
Publication date:
1978-08-05
DOI:
EISSN:
1551-5044
ISSN:
0022-1554
URN:
uuid:5a8785c8-8aeb-47e2-8586-c449ec99611c
Source identifiers:
53161
Local pid:
pubs:53161

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