Inhibition of ATP-regulated K+ channels precedes depolarization-induced increase in cytoplasmic free Ca2+ concentration in pancreatic beta-cells.

Journal article

The effects of glucose, diazoxide, K+, and tolbutamide on the activity of K+ channels, membrane potential, and cytoplasmic free Ca2+ concentration were investigated in beta-cells from the Uppsala colony of obese hyperglycemic mice. With [K+]e = [K+]i = 146 mM, it was demonstrated that the dominating channel at the resting potential is a K+ channel with a single-channel conductance of about 65 picosiemens and a reversal potential of about +70 mV (pipette potential). This channel is characteriz...

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Authors: Arkhammar, P; Nilsson, T; Rorsman, P; Berggren, P

• Publication status: Published
• Journal: Journal of biological chemistry
• Volume: 262
• Issue: 12
• Pages: 5448-5454
• Publication date: 1987-04-01
• EISSN: 1083-351X
• ISSN: 0021-9258
• Language: English
• Keywords: Islets of Langerhans; Hyperglycemia; Female; Ion Channels; Mice, Obese; Diazoxide; Animals; Male; Tolbutamide; Membrane Potentials; Calcium; Adenosine Triphosphate; Mice; Glucose; Potassium