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Journal article

Effects of aberrations and specimen structure in conventional, confocal and two-photon fluorescence microscopy.

Abstract:

Specimen-induced aberrations cause a reduction in signal levels and resolution in fluorescence microscopy. Aberrations also affect the image contrast achieved by these microscopes. We model the effects of aberrations on the fluorescence signals acquired from different specimen structures, such as point-like, linear, planar and volume structures, when imaged by conventional, confocal and two-photon microscopes. From this we derive the image contrast obtained when observing combinations of such...

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Publication status:
Published

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Institution:
University of Oxford
Department:
Oxford, MPLS, Engineering Science
Role:
Author
Journal:
Journal of microscopy
Volume:
245
Issue:
1
Pages:
63-71
Publication date:
2012-01-05
DOI:
EISSN:
1365-2818
ISSN:
0022-2720
URN:
uuid:58d6bf48-12d6-4d25-a7a9-d8fd53b22247
Source identifiers:
180424
Local pid:
pubs:180424

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