Effects of aberrations and specimen structure in conventional, confocal and two-photon fluorescence microscopy.

Journal article

Specimen-induced aberrations cause a reduction in signal levels and resolution in fluorescence microscopy. Aberrations also affect the image contrast achieved by these microscopes. We model the effects of aberrations on the fluorescence signals acquired from different specimen structures, such as point-like, linear, planar and volume structures, when imaged by conventional, confocal and two-photon microscopes. From this we derive the image contrast obtained when observing combinations of such...

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Authors: Simmonds, R; Wilson, T; Booth, M

• Publication status: Published
• Journal: Journal of microscopy
• Volume: 245
• Issue: 1
• Pages: 63-71
• Publication date: 2012-01-01
• DOI: 10.1111/j.1365-2818.2011.03544.x
• EISSN: 1365-2818
• ISSN: 0022-2720
• Language: English
• Keywords: confocal microscope; specimen structure; Aberrations; two-photon; fluorescence