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Developing promiscuous glycosidases for glycoside synthesis: residues W433 and E432 in Sulfolobus solfataricus beta-glycosidase are important glucoside- and galactoside-specificity determinants.

Abstract:

Two residues that have been implicated in determining the substrate specificity of the thermophilic beta-glycosidase from the archaeon Sulfolobus solfataricus (SsbetaG), a member of the glycosyl hydrolase family 1, have been mutated by site-directed mutagenesis so as to create more versatile catalysts for carbohydrate chemistry. The wild-type and mutated sequences were expressed in E. coli with a His(7)-tag to allow one-step chromatographic purification. The E432C and W433C mutations removed ...

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Publication status:
Published

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Publisher copy:
10.1002/cbic.200400341

Authors


Hancock, SM More by this author
Corbett, K More by this author
Fordham-Skelton, AP More by this author
Gatehouse, JA More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Organic Chemistry
Journal:
Chembiochem : a European journal of chemical biology
Volume:
6
Issue:
5
Pages:
866-875
Publication date:
2005-05-05
DOI:
EISSN:
1439-7633
ISSN:
1439-4227
URN:
uuid:588c3e1a-51c0-4aa8-bec6-6921622cb5c5
Source identifiers:
33071
Local pid:
pubs:33071

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