- Abstract:
-
Due to widespread applications of human embryonic stem (hES) cells, it is essential to establish effective protocols for cryopreservation and subsequent culture of hES cells to improve cell recovery. We have developed a new protocol for cryopreservation of dissociated hES cells and subsequent culture. We examined the effects of new formula of freezing solution containing 7.5% dimethylsulfoxide (DMSO) (v/v %) and 2.5% polyethylene glycol (PEG) (w/v %) on cell survival and recovery of hES cells...
Expand abstract - Publisher copy:
- 10.1002/btpr.358
-
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- Journal:
- Biotechnology Progress
- Volume:
- 26
- Issue:
- 3
- Pages:
- 781-788
- Publication date:
- 2010-05-05
- DOI:
- EISSN:
-
1520-6033
- ISSN:
-
8756-7938
- URN:
-
uuid:585f2f6a-2926-4aca-9474-3ec10af90c09
- Source identifiers:
-
179051
- Local pid:
- pubs:179051
- Language:
- English
- Keywords:
- Copyright date:
- 2010
Journal article
Enhancement of cell recovery for dissociated human embryonic stem cells after cryopreservation
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