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Enhancement of cell recovery for dissociated human embryonic stem cells after cryopreservation

Abstract:

Due to widespread applications of human embryonic stem (hES) cells, it is essential to establish effective protocols for cryopreservation and subsequent culture of hES cells to improve cell recovery. We have developed a new protocol for cryopreservation of dissociated hES cells and subsequent culture. We examined the effects of new formula of freezing solution containing 7.5% dimethylsulfoxide (DMSO) (v/v %) and 2.5% polyethylene glycol (PEG) (w/v %) on cell survival and recovery of hES cells...

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Publisher copy:
10.1002/btpr.358

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Journal:
Biotechnology Progress
Volume:
26
Issue:
3
Pages:
781-788
Publication date:
2010-05-01
DOI:
EISSN:
1520-6033
ISSN:
8756-7938
Source identifiers:
179051
Language:
English
Keywords:
Pubs id:
pubs:179051
UUID:
uuid:585f2f6a-2926-4aca-9474-3ec10af90c09
Local pid:
pubs:179051
Deposit date:
2013-11-16

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