Journal article icon

Journal article

Enhancement of cell recovery for dissociated human embryonic stem cells after cryopreservation

Abstract:

Due to widespread applications of human embryonic stem (hES) cells, it is essential to establish effective protocols for cryopreservation and subsequent culture of hES cells to improve cell recovery. We have developed a new protocol for cryopreservation of dissociated hES cells and subsequent culture. We examined the effects of new formula of freezing solution containing 7.5% dimethylsulfoxide (DMSO) (v/v %) and 2.5% polyethylene glycol (PEG) (w/v %) on cell survival and recovery of hES cells...

Expand abstract

Actions


Access Document


Publisher copy:
10.1002/btpr.358

Authors


Expand authors...
Journal:
Biotechnology Progress
Volume:
26
Issue:
3
Pages:
781-788
Publication date:
2010-05-05
DOI:
EISSN:
1520-6033
ISSN:
8756-7938
URN:
uuid:585f2f6a-2926-4aca-9474-3ec10af90c09
Source identifiers:
179051
Local pid:
pubs:179051

Terms of use


Metrics


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP