Pathway utilization in response to a site-specific DNA double-strand break in fission yeast.

Journal article

We have examined the genetic requirements for efficient repair of a site-specific DNA double-strand break (DSB) in Schizosaccharomyces pombe. Tech nology was developed in which a unique DSB could be generated in a non-essential minichromosome, Ch(16), using the Saccharomyces cerevisiae HO-endonuclease and its target site, MATa. DSB repair in this context was predominantly through interchromosomal gene conversion. We found that the homologous recombination (HR) genes rhp51(+), rad22A(+), rad32...

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Authors: Prudden, J; Evans, J; Hussey, S; Deans, B; O'Neill, P

• Publication status: Published
• Journal: EMBO journal
• Volume: 22
• Issue: 6
• Pages: 1419-1430
• Publication date: 2003-03-01
• DOI: 10.1093/emboj/cdg119
• EISSN: 1460-2075
• ISSN: 0261-4189
• Language: English
• Keywords: DNA, Fungal; Deoxyribonucleases, Type II Site-Specific; Fungal Proteins; Models, Biological; Schizosaccharomyces; Saccharomyces cerevisiae Proteins; Mutation; Recombination, Genetic; DNA Repair; Genes, Fungal; Saccharomyces cerevisiae; Gamma Rays; Gene Conversion; Chromosomes, Fungal; DNA Damage