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A detergent-free strategy for the reconstitution of active enzyme complexes from native biological membranes into nanoscale discs

Abstract:

Background: The reconstitution of membrane proteins and complexes into nanoscale lipid bilayer structures has contributed significantly to biochemical and biophysical analyses. Current methods for performing such reconstitutions entail an initial detergent-mediated step to solubilize and isolate membrane proteins. Exposure to detergents, however, can destabilize many membrane proteins and result in a loss of function. Amphipathic copolymers have recently been used to stabilize membrane protei...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1186/1472-6750-13-41

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Publisher:
BioMed Central Ltd. Publisher's website
Journal:
BMC Biotechnology Journal website
Volume:
13
Issue:
1
Pages:
Article: 41
Publication date:
2013-01-01
DOI:
EISSN:
1472-6750
ISSN:
1472-6750
URN:
uuid:58200249-f503-4b2f-a746-7103bc816539
Source identifiers:
413522
Local pid:
pubs:413522

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