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A soluble form of the human CD8 alpha chain expressed in the baculovirus system: biochemical characterization and binding to MHC class I.

Abstract:

We have generated a soluble form of the CD8 molecule consisting of the entire extracellular domains of the human alpha chain, by expressing a mutated CD8 alpha cDNA in SF9 cells infected with a recombinant baculovirus. The truncated molecule was secreted into the medium mostly as a disulfide-linked homodimer in which a single cysteine residue in the hinge-like region (Cys143) was sufficient to assure covalent bonding. Soluble CD8 purified to homogeneity appears to be monodisperse as assessed ...

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Publication status:
Published

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Journal:
Molecular immunology
Volume:
30
Issue:
1
Pages:
55-67
Publication date:
1993-01-05
DOI:
EISSN:
1872-9142
ISSN:
0161-5890
URN:
uuid:57bfdcbc-84f4-4e8d-8438-524e223e9d55
Source identifiers:
4928
Local pid:
pubs:4928

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