Tailoring a P. vivax vaccine to enhance efficacy through a combination of a CSP Virus-Like Particle Rv21 and TRAP viral vectors

Atcheson, E; Bauza, K; Salman, A; Alves, E; Blight, J; Viveros-Sandoval, M; Janse, C; Khan, S; Hill, A; Reyes-Sandoval, A

Journal article

Tailoring a P. vivax vaccine to enhance efficacy through a combination of a CSP Virus-Like Particle Rv21 and TRAP viral vectors

Abstract:: Vivax malaria remains one of the most serious and neglected tropical diseases, with 132 to 391 million clinical cases per year and 2.5 billion people at risk of infection. A vaccine against Plasmodium vivax could have more impact than any other intervention, and the use of a vaccine targeting multiple antigens may result in higher efficacy against sporozoite infection than targeting a single antigen. Here, two leading P. vivax preerythrocytic vaccine candidate antigens, the P. vivax circumsporozoite protein (PvCSP) and the thrombospondin-related adhesion protein (PvTRAP) were delivered as a combined vaccine. This strategy provided a dose-sparing effect, with 100% sterile protection in mice using doses that individually conferred low or no protection, as with the unadjuvanted antigens PvTRAP (0%) and PvCSP (50%), and reached protection similar to that of adjuvanted components. Efficacy against malaria infection was assessed using a new mouse challenge model consisting of a double-transgenic Plasmodium berghei parasite simultaneously expressing PvCSP and PvTRAP used in mice immunized with the virus-like particle (VLP) Rv21 previously reported to induce high efficacy in mice using Matrix-M adjuvant, while PvTRAP was concomitantly administered in chimpanzee adenovirus and modified vaccinia virus Ankara (MVA) vectors (viral-vectored TRAP, or vvTRAP) to support effective induction of T cells. We examined immunity elicited by these vaccines in the context of two adjuvants approved for human use (AddaVax and Matrix-M). Matrix-M supported the highest anti-PvCSP antibody titers when combined with Rv21, and, interestingly, mixing PvCSP Rv21 and PvTRAP viral vectors enhanced immunity to malaria over levels provided by single vaccines.

Publication status:: Published

Peer review status:: Peer reviewed

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APA Style

Atcheson, E., Bauza, K., Salman, A., Alves, E., Blight, J., Viveros-Sandoval, M., Janse, C., Khan, S., Hill, A., & Reyes-Sandoval, A. (2018). Tailoring a P. vivax vaccine to enhance efficacy through a combination of a CSP Virus-Like Particle Rv21 and TRAP viral vectors. Infection and Immunity, 86(9).

MLA Style

Atcheson, E., et al. “Tailoring a P. Vivax Vaccine to Enhance Efficacy through a Combination of a CSP Virus-Like Particle Rv21 and TRAP Viral Vectors.” Infection and Immunity, vol. 86, no. 9, American Society for Microbiology, 2018.

Chicago Style

Atcheson, E, K Bauza, A Salman, E Alves, J Blight, M Viveros-Sandoval, C Janse, S Khan, A Hill, and A Reyes-Sandoval. 2018. “Tailoring a P. Vivax Vaccine to Enhance Efficacy through a Combination of a CSP Virus-Like Particle Rv21 and TRAP Viral Vectors.” Infection and Immunity 86 (9).
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