Measurement of phosphatidylserine exposure during storage of platelet concentrates using the novel probe lactadherin: a comparison study with annexin V.

Journal article

BACKGROUND: Annexin V binding to platelets (PLTs) is considered the gold standard for monitoring phosphatidylserine (PS) exposure. However, recent comparison of annexin V with the new calcium-independent PS probe lactadherin revealed that annexin V requires a certain threshold of PS exposure (2%-8%) for binding to occur. The aim of this study was to compare annexin V and lactadherin labeling of PLTs in PLT concentrates (PCs). STUDY DESIGN AND METHODS: Optimal labeling conditions for lactadhe...

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Authors: Albanyan, A; Murphy, M; Rasmussen, J; Heegaard, C; Harrison, P

• Publication status: Published
• Journal: Transfusion
• Volume: 49
• Issue: 1
• Pages: 99-107
• Publication date: 2009-01-01
• DOI: 10.1111/j.1537-2995.2008.01933.x
• EISSN: 1537-2995
• ISSN: 0041-1132
• Language: English
• Keywords: Annexin A5; Antigens, Surface; Humans; Phosphatidylserines; P-Selectin; Preservation, Biological; Integrin beta3; Platelet Activation; Molecular Probes; Blood Platelets; Milk Proteins; Protein Binding; Platelet Glycoprotein GPIb-IX Complex