Quantitative imaging of endosome acidification and single retrovirus fusion with distinct pools of early endosomes.

Journal article

Diverse enveloped viruses enter host cells through endocytosis and fuse with endosomal membranes upon encountering acidic pH. Currently, the pH dynamics in virus-carrying endosomes and the relationship between acidification and viral fusion are poorly characterized. Here, we examined the entry of avian retrovirus that requires two sequential stimuli--binding to a cognate receptor and low pH--to undergo fusion. A genetically encoded sensor incorporated into the viral membrane was used to measu...

Expand abstract

Authors: Padilla-Parra, S; Matos, P; Kondo, N; Marin, M; Santos, N

• Journal: Proceedings of the National Academy of Sciences of the United States of America
• Volume: 109
• Issue: 43
• Pages: 17627-17632
• Publication date: 2012-10-01
• DOI: 10.1073/pnas.1211714109
• EISSN: 1091-6490
• ISSN: 0027-8424
• Language: English
• Keywords: Cell Line; Animals; Acids; Endosomes; Retroviridae; Humans; Membrane Fusion