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Infrared spectroscopy during electrocatalytic turnover reveals the Ni-L active site state during H2 oxidation by a NiFe hydrogenase.

Abstract:

We demonstrate a novel in situ infrared spectroscopic approach for the characterization of hydrogenase during catalytic turnover. E. coli hydrogenase 1 (Hyd-1) is adsorbed on a high surface-area carbon electrode and subjected to the same electrochemical control and efficient supply of substrate as in protein film electrochemistry during spectral acquisition. The spectra reveal that the active site state known as Ni-L, observed in other NiFe hydrogenases only under illumination or at cryogenic...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Accepted manuscript

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Publisher copy:
10.1002/anie.201502338

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Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Inorganic Chemistry
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Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Inorganic Chemistry
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Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Inorganic Chemistry
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Funding agency for:
Hidalgo Gonzalez, R
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Funding agency for:
Hidalgo Gonzalez, R
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Funding agency for:
Hidalgo Gonzalez, R
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Grant:
EnergyBioCatalysis-ERC-2010-StG-258600
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Publisher:
Wiley Publisher's website
Journal:
Angewandte Chemie (International ed. in English) Journal website
Volume:
54
Issue:
24
Pages:
7110-7113
Publication date:
2015-04-05
DOI:
EISSN:
1521-3773
ISSN:
1433-7851
URN:
uuid:55cd0288-01a1-4f5c-9f9a-f35d5ff22978
Source identifiers:
527965
Local pid:
pubs:527965

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