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Journal article

Replication fork blockage by transcription factor-DNA complexes in Escherichia coli.

Abstract:

All organisms require mechanisms that resuscitate replication forks when they break down, reflecting the complex intracellular environments within which DNA replication occurs. Here we show that as few as three lac repressor-operator complexes block Escherichia coli replication forks in vitro regardless of the topological state of the DNA. Blockage with tandem repressor-operator complexes was also observed in vivo, demonstrating that replisomes have a limited ability to translocate through hi...

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Publication status:
Published

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Publisher copy:
10.1093/nar/gkl682

Authors


van Knippenberg, IC More by this author
Filipe, SR More by this author
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Institution:
University of Oxford
Department:
Oxford, MSD, Biochemistry
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Journal:
Nucleic acids research
Volume:
34
Issue:
18
Pages:
5194-5202
Publication date:
2006-01-05
DOI:
EISSN:
1362-4962
ISSN:
0305-1048
URN:
uuid:5523f9dd-e5f1-428d-a1ef-5dca4e5527b0
Source identifiers:
100216
Local pid:
pubs:100216

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