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A three-way proteomics strategy allows differential analysis of yeast mitochondrial membrane protein complexes under anaerobic and aerobic conditions.

Abstract:

To investigate the effect of anaerobiosis on the Saccharomyces cerevisiae mitochondrial proteome and the formation of respiratory chain and other protein complexes, we analyzed mitochondrial protein extracts that were enriched from lysates of aerobic and anaerobic steady-state chemostat cultures. We chose an innovative approach in which native mitochondrial membrane protein complexes were separated by 1-D blue native PAGE, which was combined with quantitative analysis of each complex subunit ...

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Publication status:
Published

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Publisher copy:
10.1002/pmic.200800951

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Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Physical & Theoretical Chem
Role:
Author
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Journal:
Proteomics
Volume:
9
Issue:
20
Pages:
4787-4798
Publication date:
2009-10-01
DOI:
EISSN:
1615-9861
ISSN:
1615-9853
Source identifiers:
384587
Language:
English
Keywords:
Pubs id:
pubs:384587
UUID:
uuid:545e88ea-3bf0-4aa1-8fea-80d61ca1fe7d
Local pid:
pubs:384587
Deposit date:
2013-11-16

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