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Electrochemical and infrared spectroscopic studies provide insight into reactions of the NiFe regulatory hydrogenase from Ralstonia eutropha with O2 and CO.

Abstract:

The regulatory hydrogenase (RH) from Ralstonia eutropha acts as the H2-sensing unit of a two-component system that regulates biosynthesis of the energy conserving hydrogenases of the organism according to the availability of H2. The H2 oxidation activity, which was so far determined in vitro with artificial electron acceptors, has been considered to be insensitive to O2 and CO. It is assumed that the presence of bulky isoleucine and phenylalanine amino acid residues close to the NiFe active s...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Accepted manuscript

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Publisher copy:
10.1021/acs.jpcb.5b04164

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Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Inorganic Chemistry
Coutard, N More by this author
Heidary, N More by this author
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Funding agency for:
Vincent, KA
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Publisher:
American Chemical Society Publisher's website
Journal:
The Journal of Physical Chemistry B Journal website
Volume:
119
Issue:
43
Pages:
13807-13815
Publication date:
2015-06-05
DOI:
EISSN:
1520-5207
ISSN:
1520-6106
URN:
uuid:53bbb9bd-105d-4d6b-bcbd-644242cd2927
Source identifiers:
531624
Local pid:
pubs:531624

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