Inhibition of Na+/K(+)-ATPase may be one mechanism contributing to potassium efflux and cell shrinkage in CD95-induced apoptosis.

Journal article

To investigate the involvement of K+ efflux in apoptotic cell shrinkage, we monitored efflux of the K+ congener, 86Rb+, and cell volume during CD95-mediated apoptosis in Jurkat cells. An anti-CD95 antibody caused apoptosis associated with intracellular GSH depletion, a significant increase in 86Rb+ efflux, and a decrease in cell volume compared with control cells. Preincubating Jurkat cells with Val-Ala-Asp-chloromethylketone (VAD-cmk), an inhibitor of caspase proteases, prevented the observe...

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Authors: Nobel, C; Aronson, J; van den Dobbelsteen, D; Slater, A

• Publication status: Published
• Journal: Apoptosis : an international journal on programmed cell death
• Volume: 5
• Issue: 2
• Pages: 153-163
• Publication date: 2000-04-01
• DOI: 10.1023/a:1009684713784
• EISSN: 1573-675X
• ISSN: 1360-8185
• Language: English
• Keywords: Apoptosis; Glutathione; Sodium-Potassium-Exchanging ATPase; Antigens, CD95; Humans; Enzyme Inhibitors; Biological Transport; Potassium; Amino Acid Chloromethyl Ketones; Cell Size; Rubidium Radioisotopes; Ouabain; Jurkat Cells