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Journal article

Measurement and analysis of sarcomere length in rat cardiomyocytes in situ and in vitro.

Abstract:

Sarcomere length (SL) is an important determinant and indicator of cardiac mechanical function; however, techniques for measuring SL in living, intact tissue are limited. Here, we present a technique that uses two-photon microscopy to directly image striations of living cells in cardioplegic conditions, both in situ (Langendorff-perfused rat hearts and ventricular tissue slices, stained with the fluorescent marker di-4-ANEPPS) and in vitro (acutely isolated rat ventricular myocytes). Software...

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Publication status:
Published

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Publisher copy:
10.1152/ajpheart.00481.2009

Authors


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Institution:
University of Oxford
Department:
Oxford, MSD, Physiology Anatomy and Genetics, BHF Centre of Research Excellence
Camelliti, P More by this author
Bollensdorff, C More by this author
Stuckey, DJ More by this author
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Journal:
American journal of physiology. Heart and circulatory physiology
Volume:
298
Issue:
5
Pages:
H1616-H1625
Publication date:
2010-05-05
DOI:
EISSN:
1522-1539
ISSN:
0363-6135
URN:
uuid:53224dbf-a844-4f0f-9486-f884b304a8c7
Source identifiers:
106171
Local pid:
pubs:106171

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