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Journal article

Removal of detergents from protein extracts using activated charcoal prior to immunological analysis.

Abstract:

The use of dextran-coated activated charcoal (DCC) powder to absorb solubilising detergents from cell lysates is described. Normal embryonic epithelial cells were lysed in the presence of sodium dodecyl sulphate (SDS). The detergent was then absorbed with DCC to facilitate analysis of polycystin-1 with antibody-based methods. Polycystin-1 is a membrane protein that is involved in the pathogenesis of autosomal dominant polycystic kidney disease (ADPKD). The adverse effect of SDS on antibody-po...

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Publication status:
Published

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Institution:
University of Oxford
Division:
MSD
Department:
Pathology Dunn School
Role:
Author
Journal:
Journal of immunological methods
Volume:
264
Issue:
1-2
Pages:
37-43
Publication date:
2002-06-01
DOI:
EISSN:
1872-7905
ISSN:
0022-1759
Source identifiers:
55763
Language:
English
Keywords:
Pubs id:
pubs:55763
UUID:
uuid:53155d81-88c4-45f8-8a3d-319e27d6e6cc
Local pid:
pubs:55763
Deposit date:
2012-12-19

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