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A CRISPR Cas9 high-throughput genome editing toolkit for kinetoplastids

Abstract:

Clustered regularly interspaced short palindromic repeats (CRISPR), CRISPR-associated gene 9 (Cas9) genome editing is set to revolutionize genetic manipulation of pathogens, including kinetoplastids. CRISPR technology provides the opportunity to develop scalable methods for high-throughput production of mutant phenotypes. Here, we report development of a CRISPR-Cas9 toolkit that allows rapid tagging and gene knockout in diverse kinetoplastid species without requiring the user to perform any D...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1098/rsos.170095

Authors


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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Pathology Dunn School
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Pathology Dunn School
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Pathology Dunn School
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Pathology Dunn School
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Pathology Dunn School
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Funding agency for:
Makin, L
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Funding agency for:
Gluenz, E
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Publisher:
The Royal Society Publisher's website
Journal:
Royal Society Open Science Journal website
Volume:
4
Issue:
5
Pages:
170095
Publication date:
2017-05-03
Acceptance date:
2017-03-29
DOI:
ISSN:
2054-5703
Pubs id:
pubs:688016
URN:
uri:5250e93d-9487-4df9-8610-bc33b1c55a2b
UUID:
uuid:5250e93d-9487-4df9-8610-bc33b1c55a2b
Local pid:
pubs:688016

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