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Continuous wave excitation two-photon fluorescence microscopy exemplified with the 647-nm ArKr laser line.

Abstract:
We report on efficient two-photon fluorescence imaging in beam scanning microscopy by exciting UV dyes at the 647-nm line of a continuous wave ArKr mixed gas laser. For a numerical aperture of 1.4 (oil), we used an illumination power of up to 210 mW at the sample. High-resolution images were obtained for DAPI-labelled cell nuclei within 4-60 s. Our method is a simple two-photon alternative to UV confocal imaging with the potential of becoming a very useful feature of laser scanning microscopy.
Publication status:
Published

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Institution:
University of Oxford
Department:
Oxford, MPLS, Engineering Science
Journal:
Journal of microscopy
Volume:
190
Issue:
Pt 3
Pages:
298-304
Publication date:
1998-06-05
DOI:
EISSN:
1365-2818
ISSN:
0022-2720
URN:
uuid:52268cd2-0897-4ceb-bf11-dad9df80e9c4
Source identifiers:
64545
Local pid:
pubs:64545

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