Application of antisense technology in the control of upstream open reading frame-mediated translation

Thesis

Upstream open reading frames (uORFs) are cis-regulatory sequence elements characterized by an initiation codon located in the 5′ untranslated region (5′ UTR) and an associated in-frame termination codon located upstream or downstream of the primary ORF (pORF). uORFs have been found to inhibit translational initiation at the pORF by sequestering ribosomes through premature initiation in the 5′ UTR. It was therefore hypothesized that that the disruption of uORFs would result in increased t...

Expand abstract

Authors: Svrzikapa, N

• DOI: 10.5287/ora-o8p1w2xey
• Type of award: DPhil
• Level of award: Doctoral
• Awarding institution: University of Oxford
• Language: English
• Keywords: RNA editing; haploinsufficiency; translation; ribosome profiling; translational regulation; protein upregulation; oligonucleotides; uORF; Genetic disease; Genetic medicines
• Subjects: Drug development; Messenger RNA; Genetic disorders