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Journal article

UMI-tools: Modelling sequencing errors in Unique Molecular Identifiers to improve quantification accuracy.

Abstract:

Unique Molecular Identifiers (UMIs) are random oligonucleotide barcodes that are increasingly used in high-throughput sequencing experiments. Through a UMI, identical copies arising from distinct molecules can be distinguished from those arising through PCR-amplification of the same molecule. However, bioinformatic methods to leverage the information from UMIs have yet to be formalised. In particular, sequencing errors in the UMI sequence are often ignored, or else resolved in an ad-hoc manne...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1101/gr.209601.116

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Institution:
University of Oxford
Department:
Oxford, MSD, Physiology Anatomy and Genetics
Role:
Author
More by this author
Institution:
University of Oxford
Department:
Exeter College
Role:
Author
Publisher:
Cold Spring Harbor Laboratory Press Publisher's website
Journal:
Genome Research Journal website
Publication date:
2017-01-18
Acceptance date:
2017-01-17
DOI:
EISSN:
1549-5469
ISSN:
1088-9051
Pubs id:
pubs:673910
URN:
uri:51f1322a-cfee-4213-ad96-4b0dde31db5a
UUID:
uuid:51f1322a-cfee-4213-ad96-4b0dde31db5a
Local pid:
pubs:673910
Language:
English
Keywords:

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