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Distance measurements in the borderline region of applicability of CW EPR and DEER: a model study on a homologous series of spin-labelled peptides.

Abstract:

Inter-spin distances between 1 nm and 4.5 nm are measured by continuous wave (CW) and pulsed electron paramagnetic resonance (EPR) methods for a series of nitroxide-spin-labelled peptides. The upper distance limit for measuring dipolar coupling by the broadening of the CW spectrum and the lower distance limit for the present optimally-adjusted double electron electron resonance (DEER) set-up are determined and found to be both around 1.6-1.9 nm. The methods for determining distances and corre...

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Publication status:
Published

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Publisher copy:
10.1016/j.jmr.2007.11.023

Authors


Banham, JE More by this author
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Journal:
Journal of magnetic resonance (San Diego, Calif. : 1997)
Volume:
191
Issue:
2
Pages:
202-218
Publication date:
2008-04-05
DOI:
EISSN:
1096-0856
ISSN:
1090-7807
URN:
uuid:5179ad86-0816-4538-8893-8acac809a5e7
Source identifiers:
34134
Local pid:
pubs:34134

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