Files ending '_sorted particles' are the output of the 'iSCAMS Analysis' software. They contain a list where each row corresponds to the parameters extracted for a particles detected in the ratiometric movie. Columns correspond to parameters as follows: 1st = particle contrast 2nd = X position (pixels) 3rd = Y position (pixels) 4th = sigma X (pixels) 5th = sigma Y (pixels) 6th = offset 7th = binding time (frames) 8th = residual of 2D fit For each landing assay movie analysed, here are included the corresponding '_sorted particles' file, together with a file (.txt or .xlsx) containing a list of just the contrasts extracted.