Partial suppression of the fission yeast rqh1(-) phenotype by expression of a bacterial Holliday junction resolvase.

Journal article

A key stage during homologous recombination is the processing of the Holliday junction, which determines the outcome of the recombination reaction. To dissect the pathways of Holliday junction processing in a eukaryote, we have targeted an Escherichia coli Holliday junction resolvase to the nuclei of fission yeast recombination-deficient mutants and analysed their phenotypes. The resolvase partially complements the UV and hydroxyurea hypersensitivity and associated aberrant mitoses of an rqh1...

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Authors: Doe, C; Dixon, J; Osman, F; Whitby, M

• Publication status: Published
• Journal: EMBO journal
• Volume: 19
• Issue: 11
• Pages: 2751-2762
• Publication date: 2000-06-01
• DOI: 10.1093/emboj/19.11.2751
• EISSN: 1460-2075
• ISSN: 0261-4189
• Language: English
• Keywords: Recombination, Genetic; Luminescent Proteins; Genetic Complementation Test; Nuclear Proteins; Recombinant Fusion Proteins; Holliday Junction Resolvases; Escherichia coli; Cell Nucleus; Endodeoxyribonucleases; Schizosaccharomyces; Phenotype; DNA, Fungal; Ultraviolet Rays; Models, Genetic; Fungal Proteins; Green Fluorescent Proteins; Genes, Synthetic; Nuclear Localization Signals; Radiation Tolerance; Schizosaccharomyces pombe Proteins; Genes, Reporter; DNA Helicases; S Phase; Eukaryotic Cells; Escherichia coli Proteins