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Melittin enables efficient vesicular escape and enhanced nuclear access of nonviral gene delivery vectors.

Abstract:

Entry of exogenously applied DNA into the cytoplasm and subsequent transport into the nucleus are major cellular barriers for nonviral gene delivery vectors. To overcome these barriers, we have covalently attached the cationic peptide melittin to poly(ethylenimine) (PEI). This conjugate condensed DNA into small, discrete particles (<100 nm in diameter), and the membrane lytic activity of melittin enabled efficient release of the DNA into the cytoplasm, as monitored by fluorescence microsco...

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Publication status:
Published

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Publisher copy:
10.1074/jbc.m108331200

Authors


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Institution:
University of Oxford
Department:
Oxford, MPLS, Engineering Science
Bettinger, T More by this author
Seymour, LW More by this author
Journal:
The Journal of biological chemistry
Volume:
276
Issue:
50
Pages:
47550-47555
Publication date:
2001-12-05
DOI:
EISSN:
1083-351X
ISSN:
0021-9258
URN:
uuid:50092bd9-e360-4bf0-8ad4-b71a8bbe77da
Source identifiers:
397119
Local pid:
pubs:397119

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