- Abstract:
-
The use of methanethiosulfonates as thiol-specific modifying reagents in the strategy of combined site-directed mutagenesis and chemical modification allows virtually unlimited opportunities for creating new protein surface environments. As a consequence of our interest in electrostatic manipulation as a means of tailoring enzyme activity and specificity, we have recently adopted this approach for the controlled incorporation of multiple negative charges at single sites in the representative ...
Expand abstract - Publication status:
- Published
- Publisher copy:
- 10.1016/s0968-0896(99)00168-6
-
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- Journal:
- Bioorganic and medicinal chemistry
- Volume:
- 7
- Issue:
- 11
- Pages:
- 2303-2311
- Publication date:
- 1999-11-05
- DOI:
- EISSN:
-
1464-3391
- ISSN:
-
0968-0896
- URN:
-
uuid:4f3577d0-4f14-4480-89ec-c34e97a4bae7
- Source identifiers:
-
52041
- Local pid:
- pubs:52041
- Copyright date:
- 1999
Journal article
Altering the specificity of subtilisin Bacillus lentus through the introduction of positive charge at single amino acid sites.
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