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Altering the specificity of subtilisin Bacillus lentus through the introduction of positive charge at single amino acid sites.

Abstract:

The use of methanethiosulfonates as thiol-specific modifying reagents in the strategy of combined site-directed mutagenesis and chemical modification allows virtually unlimited opportunities for creating new protein surface environments. As a consequence of our interest in electrostatic manipulation as a means of tailoring enzyme activity and specificity, we have recently adopted this approach for the controlled incorporation of multiple negative charges at single sites in the representative ...

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Publication status:
Published

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Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Organic Chemistry
Role:
Author
Journal:
Bioorganic and medicinal chemistry
Volume:
7
Issue:
11
Pages:
2303-2311
Publication date:
1999-11-01
DOI:
EISSN:
1464-3391
ISSN:
0968-0896
Source identifiers:
52041
Language:
English
Keywords:
Pubs id:
pubs:52041
UUID:
uuid:4f3577d0-4f14-4480-89ec-c34e97a4bae7
Local pid:
pubs:52041
Deposit date:
2012-12-19

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