Journal article icon

Journal article

Altering the specificity of subtilisin Bacillus lentus through the introduction of positive charge at single amino acid sites.

Abstract:

The use of methanethiosulfonates as thiol-specific modifying reagents in the strategy of combined site-directed mutagenesis and chemical modification allows virtually unlimited opportunities for creating new protein surface environments. As a consequence of our interest in electrostatic manipulation as a means of tailoring enzyme activity and specificity, we have recently adopted this approach for the controlled incorporation of multiple negative charges at single sites in the representative ...

Expand abstract
Publication status:
Published

Actions


Access Document


Authors


More by this author
Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Organic Chemistry
Khumtaveeporn, K More by this author
Journal:
Bioorganic and medicinal chemistry
Volume:
7
Issue:
11
Pages:
2303-2311
Publication date:
1999-11-05
DOI:
EISSN:
1464-3391
ISSN:
0968-0896
URN:
uuid:4f3577d0-4f14-4480-89ec-c34e97a4bae7
Source identifiers:
52041
Local pid:
pubs:52041

Terms of use


Metrics



If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP