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Protein engineering of Bacillus megaterium CYP102. The oxidation of polycyclic aromatic hydrocarbons.

Abstract:

Cytochrome P450 (CYP) enzymes are involved in activating the carcinogenicity of polycyclic aromatic hydrocarbons (PAHs) in mammals, but they are also utilized by microorganisms for the degradation of these hazardous environmental contaminants. Wild-type CYP102 (P450(BM-3)) from Bacillus megaterium has low activity for the oxidation of the PAHs phenanthrene, fluoranthene and pyrene. The double hydrophobic substitution R47L/Y51F at the entrance of the substrate access channel increased the PAH ...

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Publication status:
Published

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Authors


Carmichael, AB More by this author
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Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Inorganic Chemistry
Journal:
European journal of biochemistry / FEBS
Volume:
268
Issue:
10
Pages:
3117-3125
Publication date:
2001-05-05
DOI:
EISSN:
1432-1033
ISSN:
0014-2956
URN:
uuid:4eff954b-10bd-407d-94b2-698a402d9330
Source identifiers:
31880
Local pid:
pubs:31880

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